Regeneration in Origanum onites by Plant Tissue Culture

Havva Atar, Hatice Çölgeçen

Abstract


The aim of this study is in vitro regeneration in Origanum onites L. is a thyme species. The semi-solid MS medium which has been used successfully in rooting studies in many plant species in vitro, has been selected. After germinating O. onites seeds in hormone-free MS medium, hypocotyls, cotyledons, epicotyl, young primary leaves and apical meristem explants were cultured in an MS medium which contains a different concentration of kinetin, a plant growth regulator. After five days, it was observed that apical meristem explants directly shoot without forming calluses. Firstly, hypocotyl, cotyledon, apical meristem, first leaf and over-ground parts developed. Lastly, root development was observed, also was the least shoot formation in the medium containing 0.5 mg L-1 kinetin and the highest shoot formation was in the medium containing 1.5 mg L-1 kinetin.

Keywords


Origanum onites, organogenesis, regeneration, in vitro

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DOI: http://dx.doi.org/10.7212%2Fzkufbd.v9i2.1237

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