An Improved Optimization Study for Determination of Pravastatin in Pharmaceutical Form by using Reversed Phase Liquid Chromatography Method

Zehra Üstün, Yaşar Doğan Daldal, Aybike Talay, Ebru Çubuk Demiralay


Pravastatin belongs to the group of anticholestrol agents used in the treatment of hypercholestaraemia. Reversed phase liquid chromatographic (RPLC) method was described in this study for its determination from tablet formulation. This method was improved with a slight change in the pH of the mobile phase. The experiment was carried out a C18 column with a mobile phase of a binary mixture of acetonitrile and water (40:60, v/v) delivered at a flow rate of 1 mL/min and detection was carried out at 239 nm. The linear range was found as 2 - 12 μg/mL. The lower of quantitation (LOQ) for pravastatin was 0.787 µg/mL. The optimization results, together with statistical evaluation of the data, demonstrated the reliability of this method.


Anticholestrol agents;Reversed Phase Liquid Chromatography (RPLC);UV detection;Pharmaceutical dosage form

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